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Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited.

Abstract : This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (gamma) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes.
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https://www.hal.inserm.fr/inserm-00391335
Contributor : Sarah Hamant <>
Submitted on : Wednesday, June 3, 2009 - 5:17:21 PM
Last modification on : Friday, November 6, 2020 - 4:02:18 AM

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  • HAL Id : inserm-00391335, version 1
  • PUBMED : 10773162

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Florence Appaix, Marie-Nathalie Minatchy, Catherine Riva-Lavieille, Jose Olivares, Bruno Antonsson, et al.. Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited.. Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 2000, 1457 (3), pp.175-81. ⟨inserm-00391335⟩

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