Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited.

Florence Appaix 1 Marie-Nathalie Minatchy 1 Catherine Riva-Lavieille 1 Jose Olivares 1 Bruno Antonsson 2 Valdur Saks 1
1 Bioénergétique fondamentale et appliquée
2 Serono Pharmaceutical Research Institute
Abstract : This paper recalls the earlier work by Keilin, Margoliash and others at the beginning of the 20th century and shows how their results can be used for the rapid solution of new problems of modern science. It describes a rapid and simple spectrophotometric method for quantitative determination of cytochrome c release from isolated mitochondria or permeabilized cells induced by proapoptotic proteins. For this, the Soret (gamma) peak at 414 nm in the spectrum of cytochrome c is used. The results of spectrophotometric assay of cytochrome c release are in accord with those of oxygraphic determination of cytochrome c-dependent respiration of isolated mitochondria and permeabilized cardiomyocytes.
Type de document :
Article dans une revue
Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 2000, 1457 (3), pp.175-81
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http://www.hal.inserm.fr/inserm-00391335
Contributeur : Sarah Hamant <>
Soumis le : mercredi 3 juin 2009 - 17:17:21
Dernière modification le : mercredi 7 novembre 2018 - 16:07:41

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  • HAL Id : inserm-00391335, version 1
  • PUBMED : 10773162

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Florence Appaix, Marie-Nathalie Minatchy, Catherine Riva-Lavieille, Jose Olivares, Bruno Antonsson, et al.. Rapid spectrophotometric method for quantitation of cytochrome c release from isolated mitochondria or permeabilized cells revisited.. Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 2000, 1457 (3), pp.175-81. 〈inserm-00391335〉

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