Skip to Main content Skip to Navigation
Journal articles

Altered mitochondrial apparent affinity for ADP and impaired function of mitochondrial creatine kinase in gluteus medius of patients with hip osteoarthritis.

Abstract : The cellular energy metabolism in human musculus gluteus medius (MGM) under normal conditions and hip osteoarthritis (OA) was explored. The functions of oxidative phosphorylation and energy transport systems were analyzed in permeabilized (skinned) muscle fibers by oxygraphy, in relation to myosin heavy chain (MHC) isoform distribution profile analyzed by SDS-PAGE, and to creatine kinase (CK) and adenylate kinase (AK) activities measured spectrophotometrically in the intact muscle. The results revealed high apparent Km for ADP in regulation of respiration that decreased after addition of creatine in MGM of traumatic patients (controls). OA was associated with increased sensitivity of mitochondrial respiration to ADP, decreased total activities of AK and CK with major reduction in mi-CK fraction, and attenuated effect of creatine on apparent Km for ADP compared with control group. It also included a complete loss of type II fibers in a subgroup of patients with the severest disease grade. It is concluded that energy metabolism in MGM cells is organized into functional complexes of mitochondria and ATPases. It is suggested that because of degenerative remodeling occurring during development of OA, these complexes become structurally and functionally impaired, which results in increased access of exogenous ADP to mitochondria and dysfunction of CK-phosphotransfer system.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00391036
Contributor : Sarah Hamant <>
Submitted on : Wednesday, June 3, 2009 - 11:43:33 AM
Last modification on : Thursday, November 19, 2020 - 3:52:36 PM

Links full text

Identifiers

Collections

LBFA | UGA

Citation

Margus Eimre, Raivo Puhke, Karin Alev, Evelin Seppet, Anneli Sikkut, et al.. Altered mitochondrial apparent affinity for ADP and impaired function of mitochondrial creatine kinase in gluteus medius of patients with hip osteoarthritis.. AJP - Regulatory, Integrative and Comparative Physiology, American Physiological Society, 2006, 290 (5), pp.R1271-5. ⟨10.1152/ajpregu.00651.2005⟩. ⟨inserm-00391036⟩

Share

Metrics

Record views

287