 |
Journal articles
An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.
Abstract : We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.
https://www.hal.inserm.fr/inserm-00390951
Contributor : Sarah Hamant <>
Submitted on : Wednesday, June 3, 2009 - 9:58:55 AM
Last modification on : Thursday, November 19, 2020 - 3:52:36 PM
Contributor : Sarah Hamant <>
Submitted on : Wednesday, June 3, 2009 - 9:58:55 AM
Last modification on : Thursday, November 19, 2020 - 3:52:36 PM
Links full text
