An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.

Uwe Riek 1 Roland Tuerk 1 Theo Wallimann 1 Uwe Schlattner 2 Dietbert Neumann 3
1 Department of Biology
2 LBFA - Bioenergétique fondamentale et appliquée
3 Cellular Stress Group
Abstract : We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.
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Article dans une revue
Biotechniques, Eaton Publishing, 2008, 45 (2), pp.187-9. 〈10.2144/000112830〉
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http://www.hal.inserm.fr/inserm-00390951
Contributeur : Sarah Hamant <>
Soumis le : mercredi 3 juin 2009 - 09:58:55
Dernière modification le : vendredi 12 juin 2009 - 14:25:46

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Uwe Riek, Roland Tuerk, Theo Wallimann, Uwe Schlattner, Dietbert Neumann. An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.. Biotechniques, Eaton Publishing, 2008, 45 (2), pp.187-9. 〈10.2144/000112830〉. 〈inserm-00390951〉

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