Creatine promotes the GABAergic phenotype in human fetal spinal cord cultures.

Angélique Ducray 1 Rachel Qualls 1 Uwe Schlattner 2, 3 Robert Andres 1 Ekkehard Dreher 4 Rolf Seiler 1 Théo Wallimann 3 Hans Rudolf Widmer 1
1 Department of Neurosurgery
2 Bioénergétique fondamentale et appliquée
3 Department of Biology
4 Department of Gynecology
Abstract : In the present study, we investigated the expression pattern of cytosolic brain specific-BB-CK and ubiquitous mitochondrial-creatine kinases (uMt-CK) in developing human spinal cord. Consequently, we studied the effects of creatine treatment on cultured fetal human spinal cord tissue. We found that both CK isoforms were expressed in fetal spinal cord at all time points investigated (5 to 11.5 weeks post conception) and correspondingly specific CK activity was detected. Chronic creatine exposure resulted in significantly higher densities of GABA-immunoreactive neurons in the cultures, while total neuronal cell density was not altered, suggesting a differentiation inducing mechanism of creatine supplementation. Taken together, our observations favour the view that the creatine phosphocreatine system plays an important role in the developing CNS.
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Article dans une revue
Brain Research, Elsevier, 2007, 1137 (1), pp.50-7. 〈10.1016/j.brainres.2006.12.038〉
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Contributeur : Sarah Hamant <>
Soumis le : mercredi 3 juin 2009 - 00:16:36
Dernière modification le : mardi 7 juillet 2009 - 12:58:45

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Angélique Ducray, Rachel Qualls, Uwe Schlattner, Robert Andres, Ekkehard Dreher, et al.. Creatine promotes the GABAergic phenotype in human fetal spinal cord cultures.. Brain Research, Elsevier, 2007, 1137 (1), pp.50-7. 〈10.1016/j.brainres.2006.12.038〉. 〈inserm-00390893〉

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