A conserved negatively charged cluster in the active site of creatine kinase is critical for enzymatic activity. - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 2000

A conserved negatively charged cluster in the active site of creatine kinase is critical for enzymatic activity.

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Abstract

Creatine kinase catalyzes the reversible transphosphorylation of creatine by MgATP. From the sequence homology and the molecular structure of creatine kinase isoenzymes, we have identified several highly conserved residues with a potential function in the active site: a negatively charged cluster (Glu(226), Glu(227), Asp(228)) and a serine (Ser(280)). Mutant proteins E226Q, E226L, E227Q, E227L, D228N, and S280A/S280D of human sarcomeric mitochondrial creatine kinase were generated by in vitro mutagenesis, expressed in Escherichia coli, and purified to homogeneity. Their overall structural integrity was confirmed by CD spectroscopy and gel filtration chromatography. The enzymatic activity of all proteins mutated in the negatively charged cluster was extremely low (0.002-0.4% of wild type) and showed apparent Michaelis constants (K(m)) similar to wild type, suggesting that most of the residual activity may be attributed to wild-type revertants. Mutations of Ser(280) led to higher residual activities and altered K(m) values; S280A showed an increase of K(m) for phosphocreatine (65-fold), creatine (6-fold), and ATP (6-fold); S280D showed a decrease of K(m) for creatine (6-fold). These results, together with the transition state structure of the homologous arginine kinase (Zhou, G., Somasundaram, T., Blanc, E., Parthasarathy G., Ellington, W. R., and Chapman, M. S. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 8449-8454), strongly suggest a critical role of Glu(226), Glu(227), and Asp(228) in substrate binding and catalysis and point to Glu(227) as a catalytic base.
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Dates and versions

inserm-00390830 , version 1 (02-06-2009)

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Michael Eder, Martin Stolz, Theo Wallimann, Uwe Schlattner. A conserved negatively charged cluster in the active site of creatine kinase is critical for enzymatic activity.. Journal of Biological Chemistry, 2000, 275 (35), pp.27094-9. ⟨10.1074/jbc.M004071200⟩. ⟨inserm-00390830⟩

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