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Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.

Abstract : Three methylated bases, 5-methylcytosine, N4-methylcytosine and N6-methyladenine (m6A), can be found in DNA. However, to date, only 5-methylcytosine has been detected in mammalian genomes. To reinvestigate the presence of m6A in mammalian DNA, we used a highly sensitive method capable of detecting one N6-methyldeoxyadenosine per million nucleosides. Our results suggest that the total mouse genome contains, if any, less than 10(3) m6A. Experiments were next performed on PRED28, a putative mammalian N6-DNA methyltransferase. The murine PRED28 encodes two alternatively spliced RNA. However, although recombinant PRED28 proteins are found in the nucleus, no evidence for an adenine-methyltransferase activity was detected.
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https://www.hal.inserm.fr/inserm-00390713
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Submitted on : Friday, July 31, 2009 - 9:31:06 AM
Last modification on : Friday, November 6, 2020 - 3:46:55 AM
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David Ratel, Jean-Luc Ravanat, Marie-Pierre Charles, Nadine Platet, Lionel Breuillaud, et al.. Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.. FEBS Letters, Wiley, 2006, 580 (13), pp.3179-84. ⟨10.1016/j.febslet.2006.04.074⟩. ⟨inserm-00390713⟩

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