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Journal articles
Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.
Abstract : Three methylated bases, 5-methylcytosine, N4-methylcytosine and N6-methyladenine (m6A), can be found in DNA. However, to date, only 5-methylcytosine has been detected in mammalian genomes. To reinvestigate the presence of m6A in mammalian DNA, we used a highly sensitive method capable of detecting one N6-methyldeoxyadenosine per million nucleosides. Our results suggest that the total mouse genome contains, if any, less than 10(3) m6A. Experiments were next performed on PRED28, a putative mammalian N6-DNA methyltransferase. The murine PRED28 encodes two alternatively spliced RNA. However, although recombinant PRED28 proteins are found in the nucleus, no evidence for an adenine-methyltransferase activity was detected.
Cited literature [14 references]
https://www.hal.inserm.fr/inserm-00390713
Contributor : Jean-Paul Issartel <>
Submitted on : Friday, July 31, 2009 - 9:31:06 AM
Last modification on : Friday, November 6, 2020 - 3:46:55 AM
Long-term archiving on: : Friday, June 11, 2010 - 12:12:51 AM
Contributor : Jean-Paul Issartel <>
Submitted on : Friday, July 31, 2009 - 9:31:06 AM
Last modification on : Friday, November 6, 2020 - 3:46:55 AM
Long-term archiving on: : Friday, June 11, 2010 - 12:12:51 AM
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