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Resistance to phorbol ester-induced differentiation in human myeloid leukemia cells: a hypothetic role for the mRNA stabilization process.

Abstract : UM384 cells, derived from the human myeloid leukemia U937 cell line, fail to differentiate in response to 12-O-tetradecanoylphorbol-13-acetate (TPA). Using cDNA microarray and real-time quantitative PCR (RT-QPCR) approaches, we observed a difference in the response to TPA treatment: all the genes from U937 cells were continuously modulated from 2 to 24h. In UM384 cells, 60% of the genes were transiently modulated at 2h, then returned to control levels at 24h. Moreover, HuR, an AU-rich element-binding protein (ARE-BP), was differentially located in the two cell lines. Therefore, a defect of mRNA stabilization could be responsible for the resistance of UM384 cells to TPA-induced differentiation, suggesting a possible role for the post-transcriptional regulation in the leukemogenesis.
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https://www.hal.inserm.fr/inserm-00390606
Contributor : Jean-Paul Issartel <>
Submitted on : Tuesday, June 2, 2009 - 2:43:55 PM
Last modification on : Thursday, November 19, 2020 - 3:56:08 PM

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Pierre Champelovier, Virginie Pautre, Michèle Elatifi, Isabelle Dupré, Béatrice Rostaing, et al.. Resistance to phorbol ester-induced differentiation in human myeloid leukemia cells: a hypothetic role for the mRNA stabilization process.. Leukemia Research, Elsevier, 2006, 30 (11), pp.1407-16. ⟨10.1016/j.leukres.2006.04.006⟩. ⟨inserm-00390606⟩

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