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Kinetic analysis of short-term effects of alpha-agonists on gluconeogenesis in isolated rat hepatocytes.

Abstract : Isolated hepatocytes from fasted rats were perifused with glycerol as gluconeogenic substrate. Stimulation of gluconeogenesis with phenylephrine (10(-5) M) as alpha-adrenergic agonist consisted of two distinct phases. The first phase was a transient stimulation of gluconeogenesis and was accompanied by transient changes in cytosolic and mitochondrial redox state; this phase was abolished by the transaminase inhibitor aminooxyacetate. The second phase was a stable stimulation of less magnitude, without change in redox state and insensitive to addition of aminooxyacetate. It is concluded that the first phase is due to a transient enhancement of flux through the malate/aspartate shuttle and that the stable phase is probably due to a stimulation of mitochondrial glycerol-3-phosphate dehydrogenase and glycerol kinase.
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https://www.hal.inserm.fr/inserm-00390283
Contributor : Sarah Hamant <>
Submitted on : Monday, June 1, 2009 - 2:57:12 PM
Last modification on : Wednesday, November 7, 2018 - 4:07:41 PM

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  • HAL Id : inserm-00390283, version 1
  • PUBMED : 2857658

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Xavier Leverve, A. K. Groen, A. J. Verhoeven, J. M. Tager. Kinetic analysis of short-term effects of alpha-agonists on gluconeogenesis in isolated rat hepatocytes.. FEBS Letters, Wiley, 1985, 181 (1), pp.43-6. ⟨inserm-00390283⟩

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