Skip to Main content Skip to Navigation
Journal articles

Control of proteolysis in perifused rat hepatocytes.

Abstract : The mechanism by means of which amino acids inhibit intrahepatic protein degradation has been studied in perifused rat hepatocytes. Proteolysis was extremely sensitive to inhibition by low concentrations of amino acids. A mixture of 0.5 mM leucine and 1-2 mM alanine, concentrations found in the portal vein of the rat after feeding, inhibited proteolysis to the same extent as a complete physiological mixture of amino acids. Inhibition by these two amino acids was accompanied by a rise in the intracellular concentrations of glutamate and aspartate, and was largely prevented by addition of glucagon, by addition of the transaminase inhibitor aminooxyacetate, or by omission of K+. Acceleration of proteolysis by K+ depletion was accompanied by a fall in intracellular glutamate caused by an increased rate of transport of this amino acid to the extracellular fluid. It is concluded that intracellular leucine, glutamate and aspartate are important elements in the control of hepatic protein degradation.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00390280
Contributor : Sarah Hamant <>
Submitted on : Monday, June 1, 2009 - 2:45:22 PM
Last modification on : Wednesday, November 7, 2018 - 4:07:41 PM

Identifiers

  • HAL Id : inserm-00390280, version 1
  • PUBMED : 3301406

Collections

Citation

Xavier Leverve, L. Heleen Caro, P. J. Plomp, Alfred Meijer. Control of proteolysis in perifused rat hepatocytes.. FEBS Letters, Wiley, 1987, 219 (2), pp.455-8. ⟨inserm-00390280⟩

Share

Metrics

Record views

597