Control of proteolysis in perifused rat hepatocytes. - Archive ouverte HAL Access content directly
Journal Articles FEBS Letters Year : 1987

Control of proteolysis in perifused rat hepatocytes.

(1) , (1) , (1) , (1)
1

Abstract

The mechanism by means of which amino acids inhibit intrahepatic protein degradation has been studied in perifused rat hepatocytes. Proteolysis was extremely sensitive to inhibition by low concentrations of amino acids. A mixture of 0.5 mM leucine and 1-2 mM alanine, concentrations found in the portal vein of the rat after feeding, inhibited proteolysis to the same extent as a complete physiological mixture of amino acids. Inhibition by these two amino acids was accompanied by a rise in the intracellular concentrations of glutamate and aspartate, and was largely prevented by addition of glucagon, by addition of the transaminase inhibitor aminooxyacetate, or by omission of K+. Acceleration of proteolysis by K+ depletion was accompanied by a fall in intracellular glutamate caused by an increased rate of transport of this amino acid to the extracellular fluid. It is concluded that intracellular leucine, glutamate and aspartate are important elements in the control of hepatic protein degradation.
Not file

Dates and versions

inserm-00390280 , version 1 (01-06-2009)

Identifiers

  • HAL Id : inserm-00390280 , version 1
  • PUBMED : 3301406

Cite

Xavier M Leverve, L. Heleen P. Caro, P. J. Plomp, Alfred J. Meijer. Control of proteolysis in perifused rat hepatocytes.. FEBS Letters, 1987, 219 (2), pp.455-8. ⟨inserm-00390280⟩

Collections

INSERM
253 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More