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Flux-dependent increase in the stoichiometry of charge translocation by mitochondrial ATPase/ATP synthase induced by almitrine.

Abstract : After studying the effects of almitrine, a new kind of ATPase/ATP synthase inhibitor, on two kinds of isolated mammalian mitochondrion, we have observed that: (1) Almitrine inhibits oligomycin-sensitive ATPase; it decreases the ATP/O value of oxidative phosphorylations without any change in the magnitude of delta mu H+. (2) Almitrine increases the mechanistic H+/ATP stoichiometry of ATPase as shown by measuring either (i) the extent of potassium acetate and of potassium phosphate accumulation sustained by ATP utilisation, or (ii) the electrical charge/ATP (K+/ATP) ratio at steady-state of ATPase activity. (3) Rat liver mitochondria are at least 10-times more sensitive to almitrine than beef heart mitochondria. (4) The change in H+/ATP stoichiometry induced by almitrine depends on the magnitude of the flux through ATPase. The inhibitory effect of almitrine on ATPase/ATP synthase complex, as a consequence of such an H+/ATP stoichiometry change, is discussed.
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https://www.hal.inserm.fr/inserm-00390274
Contributor : Sarah Hamant <>
Submitted on : Monday, June 1, 2009 - 2:35:48 PM
Last modification on : Friday, March 6, 2020 - 1:44:38 AM

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  • HAL Id : inserm-00390274, version 1
  • PUBMED : 2165421

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Michel Rigoulet, Laurent Fraisse, Rachid Ouhabi, Bernard Guérin, Eric Fontaine, et al.. Flux-dependent increase in the stoichiometry of charge translocation by mitochondrial ATPase/ATP synthase induced by almitrine.. Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 1990, 1018 (1), pp.91-7. ⟨inserm-00390274⟩

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