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Role of Na+-K+-ATPase in insulin-induced lactate release by skeletal muscle.

Abstract : Hyperinsulinemia increases lactate release by various organs and tissues. Whereas it has been shown that aerobic glycolysis is linked to Na+-K+-ATPase activity, we hypothesized that stimulation by insulin of skeletal muscle Na+-K+-ATPase is responsible for increased muscle lactate production. To test this hypothesis, we assessed muscle lactate release in healthy volunteers from the [13C]lactate concentration in the effluent dialysates of microdialysis probes inserted into the tibialis anterior muscles on both sides and infused with solutions containing 5 mmol/l [U-13C]glucose. On one side, the microdialysis probe was intermittently infused with the same solution additioned with 2.10(-5) M ouabain. In the basal state, [13C]lactate concentration in the dialysate was not affected by ouabain. During a euglycemic-hyperinsulinemic clamp, [13C]lactate concentration increased by 135% in the dialysate without ouabain, and this stimulation was nearly entirely reversed by ouabain (56% inhibition compared with values in the dialysate collected from the contralateral probe). These data indicate that insulin stimulates muscle lactate release by activating Na+-K+-ATPase in healthy humans.
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Journal articles
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Contributor : Sarah Hamant <>
Submitted on : Saturday, May 30, 2009 - 3:06:18 PM
Last modification on : Friday, November 6, 2020 - 3:46:39 AM


  • HAL Id : inserm-00390023, version 1
  • PUBMED : 11158933



Valérie Novel-Chaté, Valentine Rey, René Chioléro, Philippe Schneiter, Xavier Leverve, et al.. Role of Na+-K+-ATPase in insulin-induced lactate release by skeletal muscle.. AJP - Endocrinology and Metabolism, American Physiological Society, 2001, 280 (2), pp.E296-300. ⟨inserm-00390023⟩



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