Skip to Main content Skip to Navigation
Journal articles

Rotenone inhibits the mitochondrial permeability transition-induced cell death in U937 and KB cells.

Abstract : The permeability transition pore (PTP) is a mitochondrial inner membrane Ca(2+)-sensitive channel that plays a key role in different models of cell death. Because functional links between the PTP and the respiratory chain complex I have been reported, we have investigated the effects of rotenone on PTP regulation in U937 and KB cells. We show that rotenone was more potent than cyclosporin A at inhibiting Ca(2+)-induced PTP opening in digitonin-permeabilized cells energized with succinate. Consistent with PTP regulation by electron flux through complex I, the effect of rotenone persisted after oxidation of pyridine nucleotides by duroquinone. tert-butyl hydroperoxide induced PTP opening in intact cells (as shown by mitochondrial permeabilization to calcein and cobalt), as well as cytochrome c release and cell death. All these events were prevented by rotenone or cyclosporin A. These data demonstrate that respiratory chain complex I plays a key role in PTP regulation in vivo and confirm the importance of PTP opening in the commitment to cell death.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00389984
Contributor : Sarah Hamant <>
Submitted on : Saturday, May 30, 2009 - 1:50:04 PM
Last modification on : Friday, September 18, 2020 - 2:34:43 PM

Links full text

Identifiers

Collections

UGA | CNRS | EPHE | AGIM | PSL

Citation

Christiane Chauvin, Frédéric de Oliveira, Xavier Ronot, Mireille Mousseau, Xavier Leverve, et al.. Rotenone inhibits the mitochondrial permeability transition-induced cell death in U937 and KB cells.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (44), pp.41394-8. ⟨10.1074/jbc.M106417200⟩. ⟨inserm-00389984⟩

Share

Metrics

Record views

349