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Journal articles
Rotenone inhibits the mitochondrial permeability transition-induced cell death in U937 and KB cells.
Abstract : The permeability transition pore (PTP) is a mitochondrial inner membrane Ca(2+)-sensitive channel that plays a key role in different models of cell death. Because functional links between the PTP and the respiratory chain complex I have been reported, we have investigated the effects of rotenone on PTP regulation in U937 and KB cells. We show that rotenone was more potent than cyclosporin A at inhibiting Ca(2+)-induced PTP opening in digitonin-permeabilized cells energized with succinate. Consistent with PTP regulation by electron flux through complex I, the effect of rotenone persisted after oxidation of pyridine nucleotides by duroquinone. tert-butyl hydroperoxide induced PTP opening in intact cells (as shown by mitochondrial permeabilization to calcein and cobalt), as well as cytochrome c release and cell death. All these events were prevented by rotenone or cyclosporin A. These data demonstrate that respiratory chain complex I plays a key role in PTP regulation in vivo and confirm the importance of PTP opening in the commitment to cell death.
https://www.hal.inserm.fr/inserm-00389984
Contributor : Sarah Hamant <>
Submitted on : Saturday, May 30, 2009 - 1:50:04 PM
Last modification on : Friday, September 18, 2020 - 2:34:43 PM
Contributor : Sarah Hamant <>
Submitted on : Saturday, May 30, 2009 - 1:50:04 PM
Last modification on : Friday, September 18, 2020 - 2:34:43 PM
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