Determination of mitochondrial reactive oxygen species: methodological aspects. - Archive ouverte HAL Access content directly
Journal Articles Journal of Cellular and Molecular Medicine Year : 2002

Determination of mitochondrial reactive oxygen species: methodological aspects.

(1) , (1) , (1) , (1)
1

Abstract

The generation of Reactive Oxygen Species (ROS) as by-products in mitochondria Electron Transport Chain (ETC) has long been admitted as the cost of aerobic energy metabolism with oxidative damages as consequence. The purpose of this methodological review is to present some of the most widespread methods of ROS generation and to underline the limitations as well as some problems, identified with some experiments as examples, in the interpretation of such results. There is now no doubt that besides their pejorative role, ROS are involved in a variety of cellular processes for the continuous adaptation of the cell to its environment. Because ROS metabolism is a complex area (low production, instability of species, efficient antioxidant defense system, several places of production...) bias, variances and limitations in ROS measurements must be recognized in order to avoid artefactual conclusions, and especially to improve our understanding of physiological and pathophysiological mechanisms of such phenomenon.
Not file

Dates and versions

inserm-00389962 , version 1 (30-05-2009)

Identifiers

  • HAL Id : inserm-00389962 , version 1
  • PUBMED : 12169203

Cite

Cécile Batandier, E. Fontaine, Christiane M. Keriel, Xavier M Leverve. Determination of mitochondrial reactive oxygen species: methodological aspects.. Journal of Cellular and Molecular Medicine, 2002, 6 (2), pp.175-87. ⟨inserm-00389962⟩

Collections

INSERM UGA
39 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More