In vivo two-photon microscopy study of short-term effects of microbeam irradiation on normal mouse brain microvasculature.

Raphaël Serduc 1, * Pascale Vérant 2 Jean-Claude Vial 2 Régine Farion 1 Linda Rocas 3 Chantal Rémy 1, * Taoufik Fadlallah 3 Elke Brauer 4 Alberto Bravin 4 Jean Laissue 5 Hans Blattmann 6 Boudewijn Van Der Sanden 1
* Auteur correspondant
1 ANTE-INSERM U836, équipe 5 - Neuro-imagerie fonctionnelle et métabolique
GIN - Grenoble Institut des Neurosciences
2 LSP - Laboratoire de Spectrométrie Physique
3 Neuroimagerie Fonctionnelle et Metabolique
4 ESRF - European Synchrotron Radiation Facility
5 Oncology - Pathology - Anatomy
6 PSI - Paul Scherrer Institute
Abstract : PURPOSE: The purpose of this study was to assess the early effects of microbeam irradiation on the vascular permeability and volume in the parietal cortex of normal nude mice using two-photon microscopy and immunohistochemistry. METHODS AND MATERIALS: The upper part of the left hemisphere of 55 mice was irradiated anteroposteriorly using 18 vertically oriented beams (width 25 microm, interdistance 211 microm; peak entrance doses: 312 or 1000 Gy). At different times after microbeam exposure, the microvasculature in the cortex was analyzed using intravital two-photon microscopy after intravascular injection of fluorescein isothiocyanate (FITC)-dextrans and sulforhodamine B (SRB). Changes of the vascular volume were observed at the FITC wavelength over a maximum depth of 650 mum from the dura. The vascular permeability was detected as extravasations of SRB. RESULTS: For all times (12 h to 1 month) after microbeam irradiation and for both doses, the FITC-dextran remained in the vessels. No significant change in vascular volume was observed between 12 h and 3 months after irradiation. Diffusion of SRB was observed in microbeam irradiated regions from 12 h until 12 days only after a 1000 Gy exposure. CONCLUSION: No radiation damage to the microvasculature was detected in normal brain tissue after a 312 Gy microbeam irradiation. This dose would be more appropriate than 1000 Gy for the treatment of brain tumors using crossfired microbeams.
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Raphaël Serduc, Pascale Vérant, Jean-Claude Vial, Régine Farion, Linda Rocas, et al.. In vivo two-photon microscopy study of short-term effects of microbeam irradiation on normal mouse brain microvasculature.. International Journal of Radiation Oncology - Biology - Physics, Elsevier, 2006, 64 (5), pp.1519-27. ⟨10.1016/j.ijrobp.2005.11.047⟩. ⟨inserm-00388979⟩

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