Irradiation in presence of iodinated contrast agent results in radiosensitization of endothelial cells: consequences for computed tomography therapy.

Aurélie Joubert 1, 2 Marie-Claude Biston 1 Caroline Boudou 1 Jean-Luc Ravanat 3 Thierry Brochard 4 Anne-Marie Charvet 1 François Estève 1 Jacques Balosso 1, 5 Nicolas Foray 1
1 RSRM - Rayonnement Synchrotron et Recherche Medicale
2 IRSN - Institut de Radioprotection et de Sûreté Nucléaire
3 LAN - Laboratoire Lésions des Acides Nucléiques
SCIB - UMR E3 - Service de Chimie Inorganique et Biologique : DSM/INAC/SCIB
4 ID17 - Biomedical Beamline
5 Département de cancérologie et d'hématologie
Abstract : PURPOSE: To date, iodinated contrast agents (ICA) are commonly used in medical imaging to improve tumor visualization by attenuating scanners X-rays. However, some adverse reactions to ICAs are still reported, and their molecular origin remains unclear. In 1983, it was proposed to visualize and treat ICA-loaded tumors by using scanners as therapy machines to enhance X-rays absorption at the iodine atoms. Theoretically, such physical conditions are optimized at 50 keV and can be easily obtained with synchrotrons. METHODS AND MATERIALS: Here, we examined the molecular and cellular responses of mammalian endothelial cells to radiation in the presence of iomeprol, one of the most extensively used ICAs. RESULTS: Irradiation with X-rays at 50 keV in the presence of iomeprol produced a strong radiosensitization effect. The same conclusion was reached with a standard medical irradiator but to a lesser extent. While such treatment did not produce additional DNA double-strand breaks, we observed a dose-dependent production of iodides due to the iomeprol radiolysis that inhibit double-strand break repair rate by decreasing DNA-PK kinase activity. CONCLUSIONS: Our data suggest that the concomitant use of ICA and radiation may be toxic when radiation-produced iodide concentrations and double-strand break yields are sufficient. The potential toxicity of ICAs during X-rays for diagnosis and therapy is discussed.
Type de document :
Article dans une revue
International Journal of Radiation Oncology - Biology - Physics, Elsevier, 2005, 62 (5), pp.1486-96. 〈10.1016/j.ijrobp.2005.04.009〉
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http://www.hal.inserm.fr/inserm-00388944
Contributeur : Raphael Serduc <>
Soumis le : mercredi 27 mai 2009 - 17:24:26
Dernière modification le : mercredi 25 juillet 2018 - 16:34:01

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Aurélie Joubert, Marie-Claude Biston, Caroline Boudou, Jean-Luc Ravanat, Thierry Brochard, et al.. Irradiation in presence of iodinated contrast agent results in radiosensitization of endothelial cells: consequences for computed tomography therapy.. International Journal of Radiation Oncology - Biology - Physics, Elsevier, 2005, 62 (5), pp.1486-96. 〈10.1016/j.ijrobp.2005.04.009〉. 〈inserm-00388944〉

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