Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism.

Marie-Astrid Piquet 1, 2 Michel Roulet 3 Véronique Nogueira 2 Céline Filippi 2 Brigitte Sibille 2 Isabelle Hourmand-Ollivier 1 Marianne Pilet 3 Vincent Rouleau 4 Xavier Leverve 2
1 Département d'Hépatologie et Nutrition
2 Bioénergétique fondamentale et appliquée
3 Laboratoire de Nutrition Clinique
4 Laboratoire d'Anatomo-Pathologie
Abstract : BACKGROUND/AIMS: Polyunsaturated fatty acids (PUFA) deficiency is common in patients with alcoholic liver disease. The suitability of reversing such deficiency remains controversial. The aim was to investigate the role played by PUFA deficiency in the occurrence of alcohol-related mitochondrial dysfunction. METHODS: Wistar rats were fed either a control diet with or without alcohol (control and ethanol groups) or a PUFA deficient diet with or without alcohol (PUFA deficient and PUFA deficient+ethanol groups). After 6 weeks, liver mitochondria were isolated for energetic studies and fatty acid analysis. RESULTS: Mitochondria from ethanol fed rats showed a dramatic decrease in oxygen consumption rates and in cytochrome oxidase activity. PUFA deficiency showed an opposite picture. PUFA deficient+ethanol group roughly reach control values, regarding cytochrome oxidase activity and respiratory rates. The relationship between ATP synthesis and respiratory rate was shifted to the left in ethanol group and to the right in PUFA-deficient group. The plots of control and PUFA deficient+ethanol groups were overlapping. Phospholipid arachidonic over linoleic ratio closely correlated to cytochrome oxidase and oxygen uptake. CONCLUSIONS: PUFA deficiency reverses alcohol-related mitochondrial dysfunction via an increase in phospholipid arachidonic over linoleic ratio, which raises cytochrome oxidase activity. Such deficiency may be an adaptive mechanism.
Type de document :
Article dans une revue
Journal of Hepatology, Elsevier, 2004, 41 (5), pp.721-9. 〈10.1016/j.jhep.2004.07.002〉
Liste complète des métadonnées
http://www.hal.inserm.fr/inserm-00388772
Contributeur : Sarah Hamant <>
Soumis le : mercredi 27 mai 2009 - 13:43:15
Dernière modification le : vendredi 27 juillet 2018 - 13:42:01

Identifiants

Collections

UGA

Citation

Marie-Astrid Piquet, Michel Roulet, Véronique Nogueira, Céline Filippi, Brigitte Sibille, et al.. Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism.. Journal of Hepatology, Elsevier, 2004, 41 (5), pp.721-9. 〈10.1016/j.jhep.2004.07.002〉. 〈inserm-00388772〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

267

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD