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Towards high-throughput identification of endocrine disrupting compounds with mass spectrometry.

Abstract : High-mass matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with chemical cross-linking has the ability to monitor the ligand-dependent dimerization of the human estrogen receptor alpha ligand binding domain (hERalpha LBD) in solution. Because only ER ligands enhance the homodimer abundance, we evaluated the ability of this label-free approach for identifying endocrine disrupting compounds (EDCs) in a high-throughput manner. This was achieved by combining an automated liquid handler with an automated MS acquisition procedure, which allowed a five-fold gain in operator time compared to a fully manual approach. To detect ligand binding with enough confidence, the receptor has to be incubated with at least a 10muM concentration of the test compound. Based on the increase of the measured homodimer intensity, eight compounds with a relative binding affinity (RBA, relative to the natural hormone estradiol) >7% were identified as ER ligands among the 28 chemicals tested. Two other compounds, quercetin and 4-tert-amylphenol, were also identified as ER ligands, although their RBAs have been reported to be only 0.01% and 0.000055%, respectively. This suggests that these two ligands have a higher affinity for hERalpha LBD than reported in the literature. The high-mass MALDI approach thus allows identifying high affinity EDCs in an efficient way.
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Submitted on : Tuesday, May 26, 2009 - 11:05:23 AM
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Cédric Bovet, Benoit Plet, Marc Ruff, Sylvia Eiler, Florence Granger, et al.. Towards high-throughput identification of endocrine disrupting compounds with mass spectrometry.. Toxicology in Vitro, Elsevier, 2009, 23 (4), pp.704-9. ⟨10.1016/j.tiv.2009.02.004⟩. ⟨inserm-00387986⟩



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