Radioresistant cells expressing TLR5 control the respiratory epithelium's innate immune responses to flagellin. - Archive ouverte HAL Access content directly
Journal Articles European Journal of Immunology Year : 2009

Radioresistant cells expressing TLR5 control the respiratory epithelium's innate immune responses to flagellin.

(1) , (2) , (1) , (1) , (3) , (4) , (4) , (5) , (5) , (1) , (1)
1
2
3
4
5

Abstract

Bacterial products (such as endotoxins and flagellin) trigger innate immune responses through TLRs. Flagellin-induced signalling involves TLR5 and MyD88 and, according to some reports, TLR4. Whereas epithelial and dendritic cells are stimulated by flagellin in vitro, the cell contribution to the in vivo response is still unclear. Here, we studied the respective roles of radioresistant and radiosensitive cells in flagellin-induced airway inflammation in mice. We found that i.n. delivery of flagellin elicits a transient change in respiratory function and an acute, pro-inflammatory response in the lungs, characterized by TLR5- and MyD88-dependent chemokine secretion and neutrophil recruitment. In contrast, TLR4, CD14 and TRIF were not essential for flagellin-mediated responses, indicating that TLR4 does not cooperate with TLR5 in the lungs. Respiratory function, chemokine secretion and airway infiltration by neutrophils were dependent on radioresistant, TLR5-expressing cells. Furthermore, lung haematopoietic cells also responded to flagellin by activating TNF-alpha production. We suggest that the radioresistant lung epithelial cells are essential for initiating early, TLR5-dependent signalling in response to flagellin and thus triggering the lung's innate immune responses.
Fichier principal
Vignette du fichier
ManuscriptTLR5.pdf (3.97 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

inserm-00385299 , version 1 (18-05-2009)

Identifiers

Cite

Laure Janot, Jean-Claude Sirard, Thomas Secher, Nicolas Noulin, Lizette Fick, et al.. Radioresistant cells expressing TLR5 control the respiratory epithelium's innate immune responses to flagellin.. European Journal of Immunology, 2009, 39 (6), pp.1587-96. ⟨10.1002/eji.200838907⟩. ⟨inserm-00385299⟩
379 View
221 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More