Skip to Main content Skip to Navigation
Journal articles

Toll-like receptor 5- and lymphotoxin beta receptor-dependent epithelial Ccl20 expression involves the same NF-kappaB binding site but distinct NF-kappaB pathways and dynamics.

Abstract : Canonical and alternative NF-kappaB pathways depend on distinct NF-kappaB members and regulate expression of different gene subset in inflammatory and steady state conditions, respectively. In intestinal epithelial cells, both pathways control the transcription of the gene coding the CCL20 chemokine. Lymphotoxin beta receptor (LTbetaR) mediates long lasting CCL20 expression whereas Toll-like receptor 5 (TLR5) signals promote inducible and transient activation. Here, we investigated whether the regulation of ccl20 expression involves different promoter sites and NF-kappaB molecules in response to TLR5 and LTbetaR stimulation. In epithelial cells, both stimulation required the same promoter regions, especially the NF-kappaB binding site but involved different NF-kappaB isoforms: p65/p50 and p52/RelB, for TLR5 and LTbetaR-dependent activation, respectively. The dynamic of activation and interaction with CCL20-specific NF-kappaB site correlated with gene transcription. Similar Ccl20 expression and NF-kappaB activation was found in the small intestine of mice stimulated with TLR5 and LTbetaR agonists. In summary, different NF-kappaB pathways modulate CCL20 transcription by operating on the same NF-kappaB binding site in the same cell type.
Complete list of metadatas

Cited literature [22 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00385296
Contributor : Jean-Claude Sirard <>
Submitted on : Tuesday, May 19, 2009 - 11:35:39 AM
Last modification on : Friday, October 25, 2019 - 9:00:02 AM
Long-term archiving on: : Thursday, June 10, 2010 - 9:31:04 PM

Files

inserm-00385296_edited.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Jean-Claude Sirard, Arnaud Didierlaurent, Delphine Cayet, Frédéric Sierro, Martin Rumbo. Toll-like receptor 5- and lymphotoxin beta receptor-dependent epithelial Ccl20 expression involves the same NF-kappaB binding site but distinct NF-kappaB pathways and dynamics.. Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 2009, 1789 (5), pp.386-94. ⟨10.1016/j.bbagrm.2009.03.001⟩. ⟨inserm-00385296⟩

Share

Metrics

Record views

358

Files downloads

894