Skip to Main content Skip to Navigation
Journal articles

Functional coupling of mu-receptor-Galphai-tethered proteins in AtT20 cells.

Abstract : Opioid efficacy on mu-receptor may be influenced by various Gi/o-G-protein subunits interacting with intracellular face of receptor. Pertussis toxin-insensitive Galphai1 and Galphai2 subunits tethered with mu-receptor were stably transfected into AtT20 cells to (i) determine coupling of different alpha-subunits on opioid efficacy, and (ii) determine coupling to downstream effectors, for example, calcium and potassium channels. After pertussis toxin, stimulation of [35S]GTP-gamma-S incorporation persisted. Both constructs were able to couple to native calcium and potassium channels, with endomorphins 1 and 2 equally effective. However, pertussis toxin abolished opioid actions on calcium and potassium channels suggesting strong coupling to endogenous G-proteins, and that differences in coupling efficacy to Galphai1 and Galphai2 previously observed are restricted to initial step of signaling cascade.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00384590
Contributor : Maité Peney <>
Submitted on : Tuesday, May 26, 2009 - 4:56:44 PM
Last modification on : Thursday, April 23, 2020 - 2:26:26 PM

Identifiers

Collections

Citation

Billy Chieng, David Lee, Yan Du, Peregrine Osborne, Macdonald Christie, et al.. Functional coupling of mu-receptor-Galphai-tethered proteins in AtT20 cells.. NeuroReport, Lippincott, Williams & Wilkins, 2008, 19 (18), pp.1793-6. ⟨10.1097/WNR.0b013e3283196b55⟩. ⟨inserm-00384590⟩

Share

Metrics

Record views

241