In vivo imaging of elastic fibers using sulforhodamine B. - Archive ouverte HAL Access content directly
Journal Articles Journal of Biomedical Optics Year : 2007

In vivo imaging of elastic fibers using sulforhodamine B.

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Abstract

Until now, the imaging of elastic fibers was restricted to tissue sections using the endofluorescence properties of elastin or histological dyes. Methods to study their morphology in vivo and in situ have been lacking. We present and characterize a new application of a fluorescent dye for two-photon microscopy: sulforhodamine B (SRB), which is shown to specifically stain elastic fibers in vivo. SRB staining of elastic fibers is demonstrated to be better than using elastin endofluorescence for two-photon microscopy. Our imaging method of elastic fibers is shown to be suitable for simultaneous imaging with both other fluorescent intravital dyes and second-harmonic generation (SHG). We illustrate these findings with intravital imaging of elastic and collagen fibers in muscle epimysium and endomysium and in blood vessel walls. We expect SRB staining to become a key method to study elastic fibers in vivo.
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Dates and versions

inserm-00383878 , version 1 (13-05-2009)

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Clément Ricard, Jean-Claude Vial, Julien Douady, Boudewijn van Der Sanden. In vivo imaging of elastic fibers using sulforhodamine B.. Journal of Biomedical Optics, 2007, 12 (6), pp.064017. ⟨10.1117/1.2821421⟩. ⟨inserm-00383878⟩
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