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Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation.

Danièle Adamski 1 Jean-François Mayol 2 Nadine Platet 1 François R. Berger 1 Francis Hérodin 2 Didier Wion 1, * 
* Corresponding author
1 GIN - Grenoble Institut des Neurosciences
2 Centre de Recherches du Service de Santé des Armées
Abstract : Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data.
Keywords : Side population Hoechst 33342 cancer stem cell stem cell
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Submitted on : Friday, July 31, 2009 - 9:40:27 AM
Last modification on : Thursday, May 20, 2021 - 4:50:02 PM
Long-term archiving on: : Thursday, June 10, 2010 - 10:55:11 PM

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Danièle Adamski, Jean-François Mayol, Nadine Platet, François R. Berger, Francis Hérodin, et al.. Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation.. FEBS Letters, Wiley, 2007, 581 (16), pp.3076-80. ⟨10.1016/j.febslet.2007.05.073⟩. ⟨inserm-00382266⟩

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