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Oocyte expression with injection of purified T7 RNA polymerase.

Abstract : The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
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https://www.hal.inserm.fr/inserm-00381887
Contributor : Marco Canepari <>
Submitted on : Wednesday, June 10, 2009 - 3:00:59 PM
Last modification on : Monday, July 2, 2018 - 4:26:04 PM
Long-term archiving on: : Thursday, June 10, 2010 - 9:03:35 PM

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  • HAL Id : inserm-00381887, version 1
  • PUBMED : 16739716

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Xavier Altafaj, Nathalie Joux, Michel Ronjat, Michel de Waard. Oocyte expression with injection of purified T7 RNA polymerase.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 322, pp.55-67. ⟨inserm-00381887⟩

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