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Journal articles
Oocyte expression with injection of purified T7 RNA polymerase.
Abstract : The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
https://www.hal.inserm.fr/inserm-00381887
Contributor : Marco Canepari <>
Submitted on : Wednesday, June 10, 2009 - 3:00:59 PM
Last modification on : Monday, July 2, 2018 - 4:26:04 PM
Long-term archiving on: : Thursday, June 10, 2010 - 9:03:35 PM
Contributor : Marco Canepari <>
Submitted on : Wednesday, June 10, 2009 - 3:00:59 PM
Last modification on : Monday, July 2, 2018 - 4:26:04 PM
Long-term archiving on: : Thursday, June 10, 2010 - 9:03:35 PM
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T7-RNAP-DEWAARD-REVISED.pdf
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- Fig-1-T7RNAP.pdf Files produced by the author(s)
- Fig-2-T7RNAP.pdf Files produced by the author(s)