Oocyte expression with injection of purified T7 RNA polymerase. - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2006

Oocyte expression with injection of purified T7 RNA polymerase.

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Abstract

The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
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Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

inserm-00381887 , version 1 (10-06-2009)

Identifiers

  • HAL Id : inserm-00381887 , version 1
  • PUBMED : 16739716

Cite

Xavier Altafaj, Nathalie Joux, Michel Ronjat, Michel de Waard. Oocyte expression with injection of purified T7 RNA polymerase.. Methods in Molecular Biology, 2006, 322, pp.55-67. ⟨inserm-00381887⟩
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