Abstract : The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
https://www.hal.inserm.fr/inserm-00381887
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Submitted on : Wednesday, June 10, 2009 - 3:00:59 PM Last modification on : Friday, November 6, 2020 - 3:49:00 AM Long-term archiving on: : Thursday, June 10, 2010 - 9:03:35 PM
Xavier Altafaj, Nathalie Joux, Michel Ronjat, Michel de Waard. Oocyte expression with injection of purified T7 RNA polymerase.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 322, pp.55-67. ⟨inserm-00381887⟩