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Triadin (Trisk 95) overexpression blocks excitation-contraction coupling in rat skeletal myotubes.

Abstract : To identify the function of triadin in skeletal muscle, adenovirus-mediated overexpression of Trisk 95 or Trisk 51, the two major skeletal muscle isoforms, was induced in rat skeletal muscle primary cultures, and the physiological behavior of the modified cells was analyzed. Overexpression did not modify the expression level of their protein partners ryanodine receptor, dihydropyridine receptor, and the other triadin. Caffeine-induced calcium release was also unaffected by triadin overexpression. Nevertheless, in the absence of extracellular calcium, depolarization-induced calcium release was almost abolished in Trisk 95 overexpressing myotubes (T95 myotubes), and not modified in Trisk 51 overexpressing myotubes (T51 myotubes). This was not because of a modification of dihydropyridine receptors, as depolarization in presence of external calcium still induced a calcium release, and the activation curve of dihydropyridine receptor was unchanged, in both T95 and T51 myotubes. The calcium release complex was also maintained in T95 myotubes as Trisk 95, ryanodine receptor, dihydropyridine receptor, and Trisk 51 were still co-localized. The effect of Trisk 95 overexpression on depolarization-induced calcium release was reversed by a simultaneous infection with an antisense Trisk 95 adenovirus, indicating the specificity of this effect. Thus, the level of Trisk 95 and not Trisk 51 is important on regulating the calcium release complex, and an excess of this protein can lead to an inhibition of the physiological function of the complex.
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Submitted on : Wednesday, July 8, 2009 - 5:00:10 PM
Last modification on : Thursday, October 21, 2021 - 10:52:04 AM
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Sophia Smida Rezgui, Stéphane Vassilopoulos, Julie Brocard, Jean Claude Platel, Alexandre Bouron, et al.. Triadin (Trisk 95) overexpression blocks excitation-contraction coupling in rat skeletal myotubes.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2005, 280 (47), pp.39302-8. ⟨10.1074/jbc.M506566200⟩. ⟨inserm-00381760⟩



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