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Blood-brain barrier permeability to manganese and to Gd-DOTA in a rat model of transient cerebral ischaemia.: BBB permeability after transient cerebral ischemia

Abstract : Loss of integrity of the blood-brain barrier (BBB) and brain swelling is a potentially lethal complication of reperfusion in human stroke. To assess the time course of BBB modifications, we performed angiography, diffusion-weighted imaging, T1-weighted (T1 W) imaging and T1 mapping, and monitored acute changes after middle cerebral artery occlusion and recanalization in rats (n = 27). The animals were grouped according to the duration of occlusion: 30 min (group A, n = 8), 1 h 30 min (group B, n = 9), and 2 h 30 min (group C, n = 10). For 17 animals (four in group A, six in group B, and seven in group C), MnCl2 and dimeglumine gadoterate (Gd-DOTA) were injected at 13 min and 34 min after recanalization, respectively. The 10 remaining animals (control groups) underwent the same acquisition protocols, but no contrast agents were injected. Cell damage was determined 1 h after recanalization on haematoxylin and eosin-stained sections. Our results indicate that in the middle cerebral artery occlusion model in the rat, changes in BBB permeability assessed by contrast agent extravasation occur within the first hour of reperfusion, even after an occlusion period not exceeding 30 min. No differences between BBB permeability to Gd-DOTA and Mn2+ were detected in our experimental conditions. The reduction in apparent diffusion coefficient during occlusion appears to be a good predictor of BBB modifications after reperfusion in this model.
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https://www.hal.inserm.fr/inserm-00381344
Contributor : Michel Dojat <>
Submitted on : Monday, May 11, 2009 - 11:59:46 AM
Last modification on : Friday, November 6, 2020 - 3:49:03 AM
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Emmanuelle Grillon, Peggy Provent, Olivier Montigon, Christoph Segebarth, Chantal Rémy, et al.. Blood-brain barrier permeability to manganese and to Gd-DOTA in a rat model of transient cerebral ischaemia.: BBB permeability after transient cerebral ischemia. NMR in Biomedicine, Wiley, 2008, 21 (5), pp.427-36. ⟨10.1002/nbm.1206⟩. ⟨inserm-00381344⟩

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