Cellular disorders induced by high magnetic fields. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Journal of Magnetic Resonance Imaging Année : 2005

Cellular disorders induced by high magnetic fields.

Résumé

PURPOSE: To evaluate whether static high magnetic fields (HMFs), in the range of 10-17 T, affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons. MATERIALS AND METHODS: Cells were exposed to HMF for 30 or 60 minutes and subsequently assessed for viability. Cytoskeleton arrays and focal adhesions were visualized using immunofluorescence microscopy. RESULTS: Cell exposure to HMF over 10 T in the case of cycling cells, and over 15 T in the case of neurons, affected cell viability, apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, exposure to HMF induced growth cone retraction and delayed cell differentiation. CONCLUSION: Cell exposure to HMF (over 10T and 15 T in the case of cycling cells and neurons, respectively) affects the cell cytoskeleton, with deleterious effects on cell viability, organization, and differentiation. Further studies are needed to determine whether such perturbations, as observed here in cultured cells, have consequences in whole animals.
Fichier sous embargo
Fichier sous embargo
Date de visibilité indéterminée
Loading...

Dates et versions

inserm-00380229 , version 1 (07-05-2009)

Identifiants

Citer

Odile Valiron, Leticia Peris, Rikken G.L.J.A., Annie Schweitzer, Yasmina Saoudi, et al.. Cellular disorders induced by high magnetic fields.: High Magnetic Fields in Biology. Journal of Magnetic Resonance Imaging, 2005, 22 (3), pp.334-40. ⟨10.1002/jmri.20398⟩. ⟨inserm-00380229⟩
61 Consultations
3 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More