Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends.

Leticia Peris 1 Manuel Thery 2 Julien Fauré 1 Yasmina Saoudi 1 Laurence Lafanechère 1 John Chilton 3 Phillip Gordon-Weeks 3 Niels Galjart 4 Michel Bornens 2 Linda Wordeman 5 Juergen Wehland 6 Annie Andrieux 1, * Didier Job 1, *
* Auteur correspondant
1 Organisation Fonctionnelle du Cytosquelette
2 CDC - Compartimentation et dynamique cellulaires
3 Medical Research Council Centre for Developmental Neurobiology
4 Department of Cell Biology and Genetics
5 Department of Physiology and Biophysics
6 Department of Cell Biology
Abstract : Tubulin-tyrosine ligase (TTL), the enzyme that catalyzes the addition of a C-terminal tyrosine residue to alpha-tubulin in the tubulin tyrosination cycle, is involved in tumor progression and has a vital role in neuronal organization. We show that in mammalian fibroblasts, cytoplasmic linker protein (CLIP) 170 and other microtubule plus-end tracking proteins comprising a cytoskeleton-associated protein glycine-rich (CAP-Gly) microtubule binding domain such as CLIP-115 and p150 Glued, localize to the ends of tyrosinated microtubules but not to the ends of detyrosinated microtubules. In vitro, the head domains of CLIP-170 and of p150 Glued bind more efficiently to tyrosinated microtubules than to detyrosinated polymers. In TTL-null fibroblasts, tubulin detyrosination and CAP-Gly protein mislocalization correlate with defects in both spindle positioning during mitosis and cell morphology during interphase. These results indicate that tubulin tyrosination regulates microtubule interactions with CAP-Gly microtubule plus-end tracking proteins and provide explanations for the involvement of TTL in tumor progression and in neuronal organization.
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Article dans une revue
The Journal of Cell Biology, 2006, 174 (6), pp.839-49. 〈10.1083/jcb.200512058〉
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Leticia Peris, Manuel Thery, Julien Fauré, Yasmina Saoudi, Laurence Lafanechère, et al.. Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends.. The Journal of Cell Biology, 2006, 174 (6), pp.839-49. 〈10.1083/jcb.200512058〉. 〈inserm-00380096〉

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