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Prestress and adhesion site dynamics control cell sensitivity to extracellular stiffness.

Sophie Féréol 1 Redouane Fodil 1 Valérie Laurent 2 Martial Balland 3 Bruno Louis 1 Gabriel Pelle 4 Sylvie Hénon 3 Emmanuelle Planus 1 Daniel Isabey 1, *
* Corresponding author
1 INSERM U955, équipe 13
IMRB - Institut Mondor de Recherche Biomédicale
2 INSERM U955, équipe 13
LIPhy [2011-2015] - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] [2011-2015], IMRB - Institut Mondor de Recherche Biomédicale
4 INSERM U955, équipe 13
Service de physiologie, explorations fonctionnelles [Mondor], IMRB - Institut Mondor de Recherche Biomédicale
Abstract : This study aims at improving the understanding of mechanisms responsible for cell sensitivity to extracellular environment. We explain how substrate mechanical properties can modulate the force regulation of cell sensitive elements primarily adhesion sites. We present a theoretical and experimental comparison between two radically different approaches of the force regulation of adhesion sites that depends on their either stationary or dynamic behavior. The most classical stationary model fails to predict cell sensitivity to substrate stiffness whereas the dynamic model predicts extracellular stiffness dependence. This is due to a time dependent reaction force in response to actomyosin traction force exerted on cell sensitive elements. We purposely used two cellular models, i.e., alveolar epithelial cells and alveolar macrophages exhibiting respectively stationary and dynamic adhesion sites, and compared their sensitivity to theoretical predictions. Mechanical and structural results show that alveolar epithelial cells exhibit significant prestress supported by evident stress fibers and lacks sensitivity to substrate stiffness. On the other hand, alveolar macrophages exhibit low prestress and exhibit sensitivity to substrate stiffness. Altogether, theory and experiments consistently show that adhesion site dynamics and cytoskeleton prestress control cell sensitivity to extracellular environment with an optimal sensitivity expected in the intermediate range.
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https://www.hal.inserm.fr/inserm-00371938
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Submitted on : Monday, March 30, 2009 - 5:39:57 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:03 AM
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Sophie Féréol, Redouane Fodil, Valérie Laurent, Martial Balland, Bruno Louis, et al.. Prestress and adhesion site dynamics control cell sensitivity to extracellular stiffness.. Biophysical Journal, Biophysical Society, 2009, 96 (5), pp.2009-22. ⟨10.1016/j.bpj.2008.10.072⟩. ⟨inserm-00371938⟩

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