Restoration of the crystallization of altered delta-endotoxins Cry1Ac, by the promotion of their in vivo integration into the Bacillus thuringiensis native crystals. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue FEMS Microbiology Letters Année : 2009

Restoration of the crystallization of altered delta-endotoxins Cry1Ac, by the promotion of their in vivo integration into the Bacillus thuringiensis native crystals.

Résumé

Cry1Ac is one of the most-studied Bacillus thuringiensisdelta-endotoxins. Structurally, it is divided into two domains: the N-terminal half corresponding to the toxic component and the C-terminal half corresponding to the region responsible for the crystal formation. We engineered Cry1Ac delta-endotoxins modified in their N-terminal part and studied the effect of such modifications on crystallization and delta-endotoxin production. When expressed in an acrystalliferous B. thuringiensis strain, Cry1Ac(*) and Cry1AcDelta, variants with four point mutations and a deletion, respectively, could not form crystals. However, when expressed in a crystalliferous strain, these altered proteins were shown to interact with the endogenous delta-endotoxins and cocrystallize with them, forming atypical crystals observed by electron microscopy. This cocrystallization of the altered delta-endotoxins with the endogenous ones led to a decrease in delta-endotoxin production (27%) by the corresponding recombinant B. thuringiensis strains. This ability of altered delta-endotoxins containing an intact C-terminal part to cocrystallize with native ones could be exploited to promote the crystallization of foreign proteins by fusing them with the C-terminal part of Cry1A delta-endotoxins.

Dates et versions

inserm-00370116 , version 1 (23-03-2009)

Identifiants

Citer

Mariam Dammak, Slim Tounsi, Dorra Ben Hamadou, Lobna Abdelkafi, Patrick Schultz, et al.. Restoration of the crystallization of altered delta-endotoxins Cry1Ac, by the promotion of their in vivo integration into the Bacillus thuringiensis native crystals.. FEMS Microbiology Letters, 2009, 292 (2), pp.268-73. ⟨10.1111/j.1574-6968.2009.01493.x⟩. ⟨inserm-00370116⟩
461 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More