Skip to Main content Skip to Navigation
Journal articles

Restoration of the crystallization of altered delta-endotoxins Cry1Ac, by the promotion of their in vivo integration into the Bacillus thuringiensis native crystals.

Abstract : Cry1Ac is one of the most-studied Bacillus thuringiensisdelta-endotoxins. Structurally, it is divided into two domains: the N-terminal half corresponding to the toxic component and the C-terminal half corresponding to the region responsible for the crystal formation. We engineered Cry1Ac delta-endotoxins modified in their N-terminal part and studied the effect of such modifications on crystallization and delta-endotoxin production. When expressed in an acrystalliferous B. thuringiensis strain, Cry1Ac(*) and Cry1AcDelta, variants with four point mutations and a deletion, respectively, could not form crystals. However, when expressed in a crystalliferous strain, these altered proteins were shown to interact with the endogenous delta-endotoxins and cocrystallize with them, forming atypical crystals observed by electron microscopy. This cocrystallization of the altered delta-endotoxins with the endogenous ones led to a decrease in delta-endotoxin production (27%) by the corresponding recombinant B. thuringiensis strains. This ability of altered delta-endotoxins containing an intact C-terminal part to cocrystallize with native ones could be exploited to promote the crystallization of foreign proteins by fusing them with the C-terminal part of Cry1A delta-endotoxins.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00370116
Contributor : Maité Peney <>
Submitted on : Monday, March 23, 2009 - 3:54:37 PM
Last modification on : Thursday, April 23, 2020 - 2:26:26 PM

Links full text

Identifiers

Collections

Citation

Mariam Dammak, Slim Tounsi, Dorra Ben Hamadou, Lobna Abdelkafi, Patrick Schultz, et al.. Restoration of the crystallization of altered delta-endotoxins Cry1Ac, by the promotion of their in vivo integration into the Bacillus thuringiensis native crystals.. FEMS Microbiology Letters, Wiley-Blackwell, 2009, 292 (2), pp.268-73. ⟨10.1111/j.1574-6968.2009.01493.x⟩. ⟨inserm-00370116⟩

Share

Metrics

Record views

981