 |
Journal articles
E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35.
Abstract : The transcription factor E2F1 has a key function during S phase progression and apoptosis. It has been well-demonstrated that the apoptotic function of E2F1 involves its ability to transactivate pro-apoptotic target genes. Alternative splicing of pre-mRNAs also has an important function in the regulation of apoptosis. In this study, we identify the splicing factor SC35, a member of the Ser-Rich Arg (SR) proteins family, as a new transcriptional target of E2F1. We demonstrate that E2F1 requires SC35 to switch the alternative splicing profile of various apoptotic genes such as c-flip, caspases-8 and -9 and Bcl-x, towards the expression of pro-apoptotic splice variants. Finally, we provide evidence that E2F1 upregulates SC35 in response to DNA-damaging agents and show that SC35 is required for apoptosis in response to these drugs. Taken together, these results demonstrate that E2F1 controls pre-mRNA processing events to induce apoptosis and identify the SC35 SR protein as a key direct E2F1-target in this setting.
https://www.hal.inserm.fr/inserm-00360967
Contributor : Christian Brambilla <>
Submitted on : Tuesday, February 17, 2009 - 4:52:01 PM
Last modification on : Friday, November 6, 2020 - 3:50:34 AM
Contributor : Christian Brambilla <>
Submitted on : Tuesday, February 17, 2009 - 4:52:01 PM
Last modification on : Friday, November 6, 2020 - 3:50:34 AM
Links full text
