Skip to Main content Skip to Navigation
Journal articles

Disruption of the interaction between transcriptional intermediary factor 1{beta} and heterochromatin protein 1 leads to a switch from DNA hyper- to hypomethylation and H3K9 to H3K27 trimethylation on the MEST promoter correlating with gene reactivation.: TIF1beta–HP1 Association Functions in MEST Repression

Abstract : Here, we identified the imprinted mesoderm-specific transcript (MEST) gene as an endogenous TIF1beta primary target gene and demonstrated that transcriptional intermediary factor (TIF) 1beta, through its interaction with heterochromatin protein (HP) 1, is essential in establishing and maintaining a local heterochromatin-like structure on MEST promoter region characterized by H3K9 trimethylation and hypoacetylation, H4K20 trimethylation, DNA hypermethylation, and enrichment in HP1 that correlates with preferential association to foci of pericentromeric heterochromatin and transcriptional repression. On disruption of the interaction between TIF1beta and HP1, TIF1beta is released from the promoter region, and there is a switch from DNA hypermethylation and histone H3K9 trimethylation to DNA hypomethylation and histone H3K27 trimethylation correlating with rapid reactivation of MEST expression. Interestingly, we provide evidence that the imprinted MEST allele DNA methylation is insensitive to TIF1beta loss of function, whereas the nonimprinted allele is regulated through a distinct TIF1beta-DNA methylation mechanism.
Document type :
Journal articles
Complete list of metadatas

Cited literature [46 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00357465
Contributor : Maité Peney <>
Submitted on : Tuesday, October 26, 2010 - 5:31:16 PM
Last modification on : Thursday, April 23, 2020 - 2:26:26 PM
Long-term archiving on: : Thursday, January 27, 2011 - 2:28:17 AM

File

296.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Raphaël Riclet, Mariam Chendeb, Jean-Luc Vonesch, Dirk Koczan, Hans-Juergen Thiesen, et al.. Disruption of the interaction between transcriptional intermediary factor 1{beta} and heterochromatin protein 1 leads to a switch from DNA hyper- to hypomethylation and H3K9 to H3K27 trimethylation on the MEST promoter correlating with gene reactivation.: TIF1beta–HP1 Association Functions in MEST Repression. Molecular Biology of the Cell, American Society for Cell Biology, 2009, 20 (1), pp.296-305. ⟨10.1091/mbc.E08-05-0510⟩. ⟨inserm-00357465⟩

Share

Metrics

Record views

551

Files downloads

1167