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A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.

Abstract : We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs.
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https://www.hal.inserm.fr/inserm-00357392
Contributor : Maité Peney <>
Submitted on : Friday, January 30, 2009 - 11:20:01 AM
Last modification on : Thursday, April 23, 2020 - 2:26:26 PM

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Wassim Abdulrahman, Muriel Uhring, Isabelle Kolb-Cheynel, Jean-Marie Garnier, Dino Moras, et al.. A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.. Analytical Biochemistry, Elsevier Masson, 2009, 385 (2), pp.383-5. ⟨10.1016/j.ab.2008.10.044⟩. ⟨inserm-00357392⟩

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