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Journal Articles Analytical Biochemistry Year : 2009

A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.

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Abstract

We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs.
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Dates and versions

inserm-00357392 , version 1 (30-01-2009)

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Wassim Abdulrahman, Muriel Uhring, Isabelle Kolb-Cheynel, Jean-Marie Garnier, Dino Moras, et al.. A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.. Analytical Biochemistry, 2009, 385 (2), pp.383-5. ⟨10.1016/j.ab.2008.10.044⟩. ⟨inserm-00357392⟩
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