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Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse.

Abstract : To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER(T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders.
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https://www.hal.inserm.fr/inserm-00357169
Contributor : Maité Peney <>
Submitted on : Thursday, January 29, 2009 - 4:31:06 PM
Last modification on : Thursday, April 23, 2020 - 2:26:35 PM

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Olivia Wendling, Jean-Marc Bornert, Pierre Chambon, Daniel Metzger. Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse.. Genesis, Wiley-Blackwell, 2009, 47 (1), pp.14-8. ⟨10.1002/dvg.20448⟩. ⟨inserm-00357169⟩

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