ElrA and AUF1 differentially bind cyclin B2 mRNA. - Archive ouverte HAL Access content directly
Journal Articles Biochemical and Biophysical Research Communications Year : 2008

ElrA and AUF1 differentially bind cyclin B2 mRNA.

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Abstract

In Xenopus embryos, maternal cyclins drive the first 12 cell divisions after which several cyclins are terminally degraded, including cyclin B2. Cyclin B2 disappearance is due to transcription-mediated mRNA deadenylation at the midblastula transition, when transcription initiates and the cell cycle lengthens. To further define the mechanism, we characterized proteins capable of binding cyclin B2 3'UTR. We show that ElrA and AUF1 compete for binding to regions containing cytoplasmic polyadenylation elements (CPEs), with AUF1 binding increasing at the midblastula transition. Deletion of both CPEs abrogates polyadenylation but has no effect on deadenylation or binding of ElrA or AUF1. Overexpression of ElrA or AUF1 does not alter cyclin B2 mRNA stability. These results show that ElrA and AUF1 bind to cyclin B2 mRNA independent of CPEs and function by binding other elements.

Dates and versions

inserm-00353157 , version 1 (14-01-2009)

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Xun Guo, Francoise Gourronc, Yann Audic, Gillian Lyons-Levy, Therese Mitchell, et al.. ElrA and AUF1 differentially bind cyclin B2 mRNA.. Biochemical and Biophysical Research Communications, 2008, 377 (2), pp.653-7. ⟨10.1016/j.bbrc.2008.10.029⟩. ⟨inserm-00353157⟩
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