Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst. - Archive ouverte HAL Access content directly
Journal Articles Developmental Biology Year : 2008

Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst.

(1) , (2, 3) , (2, 3) , (1)
1
2
3

Abstract

Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE cells are determined within the blastocyst ICM long before epithelium formation. In this work, we isolated Lrp2 as a novel PrE precursor (pre-PrE) marker by using a microarray strategy that combines a transcriptome analysis of three stem cell lines and early embryos. A detailed expression analysis shows that Lrp2 expression is induced in late E3.5 embryos indicating that pre-PrE cells are progressively maturing prior to polarization into an epithelium. Furthermore, the subcellular location of Lrp2, Disabled-2 (Dab2) and Collagen-IV shows that the epithelial structure is acquired in individual cells through successive steps.
Fichier principal
Vignette du fichier
Gerbe_et_al.pdf (688.84 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

inserm-00352675 , version 1 (13-01-2009)

Identifiers

Cite

François Gerbe, Brian Cox, Janet Rossant, Claire Chazaud. Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst.. Developmental Biology, 2008, 313 (2), pp.594-602. ⟨10.1016/j.ydbio.2007.10.048⟩. ⟨inserm-00352675⟩
155 View
485 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More