The words of the regulatory code are arranged in a variable manner in highly conserved enhancers. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Developmental Biology Année : 2008

The words of the regulatory code are arranged in a variable manner in highly conserved enhancers.

Résumé

The cis-regulatory regions of many developmental regulators and transcription factors are believed to be highly conserved in the genomes of vertebrate species, suggesting specific regulatory mechanisms for these gene classes. We functionally characterized five notochord enhancers, whose sequence is highly conserved, and systematically mutated two of them. Two subregions were identified to be essential for expression in the notochord of the zebrafish embryo. Synthetic enhancers containing the two essential regions in front of a TATA-box drive expression in the notochord while concatemerization of the subregions alone is not sufficient, indicating that the combination of the two sequence elements is required for notochord expression. Both regions are present in the five functionally characterized notochord enhancers. However, the position, the distance and relative orientation of the two sequence motifs can vary substantially within the enhancer sequences. This suggests that the regulatory grammar itself does not dictate the high evolutionary conservation between these orthologous cis-regulatory sequences. Rather, it represents a less well-conserved layer of sequence organization within these sequences.

Domaines

Ethique

Dates et versions

inserm-00351017 , version 1 (08-01-2009)

Identifiants

Citer

Sepand Rastegar, Isabell Hess, Thomas Dickmeis, Jean Christophe Nicod, Raymond Ertzer, et al.. The words of the regulatory code are arranged in a variable manner in highly conserved enhancers.. Developmental Biology, 2008, 318 (2), pp.366-77. ⟨10.1016/j.ydbio.2008.03.034⟩. ⟨inserm-00351017⟩
419 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More