Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii. - Archive ouverte HAL Access content directly
Journal Articles Acta crystallographica Section F : Structural biology communications [2014-...] Year : 2008

Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii.

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Satoru Shimizu
  • Function : Author
Ella Czarina Magat Juan
  • Function : Author
Yu Ichiro Miyashita
  • Function : Author
Yoshiteru Sato
  • Function : Author
Md Mominul Hoque
  • Function : Author
Kaoru Suzuki
  • Function : Author
Masataka Yogiashi
  • Function : Author
Masaru Tsunoda
  • Function : Author
Takeshi Sekiguchi
  • Function : Author
Akio Takénaka
  • Function : Author

Abstract

Threonyl-tRNA synthetase (ThrRS) plays an essential role in protein synthesis by catalyzing the aminoacylation of tRNA(Thr) and editing misacylation. ThrRS generally contains an N-terminal editing domain, a catalytic domain and an anticodon-binding domain. The sequences of the editing domain in ThrRSs from archaea differ from those in bacteria and eukaryotes. Furthermore, several creanarchaea including Aeropyrum pernix K1 and Sulfolobus tokodaii strain 7 contain two genes encoding either the catalytic or the editing domain of ThrRS. To reveal the structural basis for this evolutionary divergence, the two types of ThrRS from the crenarchaea A. pernix and S. tokodaii have been overexpressed in Eschericha coli, purified and crystallized by the hanging-drop vapour-diffusion method. Diffraction data were collected and the structure of a selenomethionine-labelled A. pernix type-1 ThrRS crystal has been solved using the MAD method.

Dates and versions

inserm-00350840 , version 1 (03-02-2009)

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Satoru Shimizu, Ella Czarina Magat Juan, Yu Ichiro Miyashita, Yoshiteru Sato, Md Mominul Hoque, et al.. Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii.. Acta crystallographica Section F : Structural biology communications [2014-..], 2008, 64 (Pt 10), pp.903-10. ⟨10.1107/S1744309108026924⟩. ⟨inserm-00350840⟩
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