STRA8-deficient spermatocytes initiate, but fail to complete, meiosis and undergo premature chromosome condensation. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2008

STRA8-deficient spermatocytes initiate, but fail to complete, meiosis and undergo premature chromosome condensation.

Résumé

We analysed the phenotypic outcome of a Stra8-null mutation on male meiosis. Because the mutant spermatocytes (1) underwent premeiotic DNA replication, (2) displayed cytological features attesting initiation of recombination and of axial-element assembly, and (3) expressed Spo11 and numerous other meiotic genes, it was concluded that STRA8 is dispensable for meiotic initiation. The few mutant spermatocytes that progressed beyond leptonema showed a prolonged bouquet-stage configuration, asynapsis and heterosynapsis, suggesting function(s) of STRA8 in chromosome pairing. Most importantly, a large number of mutant leptotene spermatocytes underwent premature chromosome condensation, within 24 hours following the meiotic S phase. This phenomenon yielded aberrant metaphase-like cells with 40 univalent chromosomes, similar to normal mitotic metaphases. From these latter observations and from the wild-type pattern of Stra8 expression, we propose that, in preleptotene spermatocytes, STRA8 is involved in the process that leads to stable commitment to the meiotic cell cycle.
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Dates et versions

inserm-00350807 , version 1 (07-01-2009)

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Manuel Mark, Hugues Jacobs, Mustapha Oulad-Abdelghani, Christine Dennefeld, Betty Féret, et al.. STRA8-deficient spermatocytes initiate, but fail to complete, meiosis and undergo premature chromosome condensation.. Journal of Cell Science, 2008, 121 (Pt 19), pp.3233-42. ⟨10.1242/jcs.035071⟩. ⟨inserm-00350807⟩
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