Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Journal of Neuroscience Research Année : 2008

Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro.

Philippe Sabatier
  • Fonction : Auteur
  • PersonId : 852580
Hugues Duffau
Monica Prieto
  • Fonction : Auteur
Manuel Gaviria
  • Fonction : Auteur

Résumé

Adult human and rodent brains contain neural stem and progenitor cells, and the presence of neural stem cells in the adult rodent spinal cord has also been described. Here, using electron microscopy, expression of neural precursor cell markers, and cell culture, we investigated whether neural precursor cells are also present in adult human spinal cord. In well-preserved nonpathological post-mortem human adult spinal cord, nestin, Sox2, GFAP, CD15, Nkx6.1, and PSA-NCAM were found to be expressed heterogeneously by cells located around the central canal. Ultrastructural analysis revealed the existence of immature cells close to the ependymal cells, which display characteristics of type B and C cells found in the adult rodent brain subventricular region, which are considered to be stem and progenitor cells, respectively. Completely dissociated spinal cord cells reproducibly formed Sox2(+) nestin(+) neurospheres containing proliferative precursor cells. On differentiation, these generate glial cells and gamma-aminobutyric acid (GABA)-ergic neurons. These results provide the first evidence for the existence in the adult human spinal cord of neural precursors with the potential to differentiate into neurons and glia. They represent a major interest for endogenous regeneration of spinal cord after trauma and in degenerative diseases.

Dates et versions

inserm-00349495 , version 1 (06-01-2009)

Identifiants

Citer

Cécile Dromard, Hélène Guillon, Valérie Rigau, Chantal Ripoll, Jean-Charles Sabourin, et al.. Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro.. Journal of Neuroscience Research, 2008, 86 (9), pp.1916-26. ⟨10.1002/jnr.21646⟩. ⟨inserm-00349495⟩
195 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More