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Beta-lactam induction of ISEcp1B-mediated mobilization of the naturally occurring bla(CTX-M) beta-lactamase gene of Kluyvera ascorbata.

Abstract : ISEcp1B is an insertion element associated with the emerging expanded-spectrum beta-lactamase bla(CTX-M) genes in Enterobacteriaceae. Because ISEcp1B-bla(CTX-M)positive strains may be identified from humans and animals, the ability of this insertion sequence to mobilize the bla(CTX-M-2) gene was tested from its progenitor Kluyvera ascorbata to study the effects of amoxicillin/clavulanic and cefquinome as enhancers of transposition. These beta-lactam molecules are administered parenterally to treat infected animals. ISEcp1B-mediated mobilization of the bla(CTX-M-2) gene from K. ascorbata to a plasmid location in Escherichia coli J53 was studied. Transposition assays were performed with overnight cultures with amoxicillin/clavulanic acid and cefquinome at concentrations expected to mimic those found in feces after parenteral administration (0.4-0.008 mg L(-1) and 0.32-0.064 mg L(-1), respectively). Amoxicillin/clavulanic acid and cefquinome did not modify the transposition frequency (1.85+/-1.7 x 10(-7)) whereas ceftazidime (0.5 mg L(-1)), used as a control, did (5.2+/-2.7 x 10(-5)). Therefore, it is likely that neither amoxicillin/clavulanic acid nor cefquinome concentrations as found in the gut flora may enhance mobilization of the bla(CTX-M) genes in Enterobacteriaceae.
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https://www.hal.inserm.fr/inserm-00347198
Contributor : Patrice Nordmann <>
Submitted on : Monday, December 15, 2008 - 10:24:48 AM
Last modification on : Tuesday, June 23, 2020 - 11:38:01 AM
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Patrice Nordmann, Marie-Frédérique Lartigue, Laurent Poirel. Beta-lactam induction of ISEcp1B-mediated mobilization of the naturally occurring bla(CTX-M) beta-lactamase gene of Kluyvera ascorbata.. FEMS Microbiology Letters, Wiley-Blackwell, 2008, 288 (2), pp.247-9. ⟨10.1111/j.1574-6968.2008.01359.x⟩. ⟨inserm-00347198⟩

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