Skip to Main content Skip to Navigation
Journal articles

In vivo measurement of lung capillary-alveolar macromolecule permeability by saturation bronchoalveolar lavage.

Sam Bayat 1 Daniel Anglade 1 Ahmed Menaouar 2 Jean-Louis Martiel 3 Jean-Luc Lafond 4 Gila Benchetrit 1 Francis Grimbert 1
1 TIMC-PRETA [?-2015] - Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée [?-2015]
TIMC [2011-2015] - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 [2011-2015]
3 TIMB
TIMC [2011-2015] - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 [2011-2015]
Abstract : OBJECTIVE: Measurement of capillary-alveolar permeability to fluorescein isothiocyanate-dextran (FITC-D) (molecular mass, 71,300 daltons) by a sequential bronchoalveolar lavage (BAL) technique. DESIGN: Animal research. SETTING: The Department of Physiology at a scientific and medical university. SUBJECTS: Nine anesthetized and mechanically ventilated dogs. INTERVENTIONS: Two separate experiments were performed in each subject-an initial control experiment followed by an oleic acid-induced lung injury. The indicator was administered at constant blood concentration before serial BAL including eight fluid instillation-recovery cycles. MEASUREMENTS: Plasma to BAL solute clearance at saturation (capillary-alveolar clearance at saturation, mL/min) was calculated and normalized to lavage fluid volume (measured by 1251 serum albumin dilution) to obtain a transport rate (TR) constant. MAIN RESULTS: TR for FITC-D70 was 4.0+/-0.8 and 46.1+/-18.1 x 10(-5) x min(-1) in control and injured lung, respectively (p < .02). Capillary-alveolar clearance of FITC-D70 was not affected by the lavage procedure itself. TR reflected essentially epithelial permeability in normal lung and combined epithelial and endothelial permeability in injured lung. A significant correlation was found between cardiac output and TR in injured lung. CONCLUSIONS: Saturation BAL allowed us to estimate capillary-alveolar macromolecule permeability in vivo in dogs. Further study may allow bedside evaluation of lung injury by BAL in patients.
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00338174
Contributor : Jean-Louis Martiel <>
Submitted on : Tuesday, November 11, 2008 - 6:51:08 PM
Last modification on : Friday, July 17, 2020 - 2:06:14 PM

Identifiers

  • HAL Id : inserm-00338174, version 1
  • PUBMED : 10966275

Collections

Citation

Sam Bayat, Daniel Anglade, Ahmed Menaouar, Jean-Louis Martiel, Jean-Luc Lafond, et al.. In vivo measurement of lung capillary-alveolar macromolecule permeability by saturation bronchoalveolar lavage.. Critical Care Medicine, Lippincott, Williams & Wilkins, 2000, 28 (8), pp.2937-42. ⟨inserm-00338174⟩

Share

Metrics

Record views

282