Skip to Main content Skip to Navigation
Journal articles

Single nucleotide polymorphisms, apoptosis, and the development of severe late adverse effects after radiotherapy.

Abstract : PURPOSE: Evidence has accumulated in recent years suggestive of a genetic basis for a susceptibility to the development of radiation injury after cancer radiotherapy. The purpose of this study was to assess whether patients with severe radiation-induced sequelae (RIS; i.e., National Cancer Institute/CTCv3.0 grade, > or =3) display both a low capacity of radiation-induced CD8 lymphocyte apoptosis (RILA) in vitro and possess certain single nucleotide polymorphisms (SNP) located in candidate genes associated with the response of cells to radiation. EXPERIMENTAL DESIGN: DNA was isolated from blood samples obtained from patients (n = 399) included in the Swiss prospective study evaluating the predictive effect of in vitro RILA and RIS. SNPs in the ATM, SOD2, XRCC1, XRCC3, TGFB1, and RAD21 genes were screened in patients who experienced severe RIS (group A, n = 16) and control subjects who did not manifest any evidence of RIS (group B, n = 18). RESULTS: Overall, 13 and 21 patients were found to possess a total of <4 and > or =4 SNPs in the candidate genes. The median (range) RILA in group A was 9.4% (5.3-16.5) and 94% (95% confidence interval, 70-100) of the patients (15 of 16) had > or =4 SNPs. In group B, median (range) RILA was 25.7% (20.2-43.2) and 33% (95% confidence interval, 13-59) of patients (6 of 18) had > or =4 SNPs (P < 0.001). CONCLUSIONS: The results of this study suggest that patients with severe RIS possess 4 or more SNPs in candidate genes and low radiation-induced CD8 lymphocyte apoptosis in vitro.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00337542
Contributor : Yves Le Ster <>
Submitted on : Thursday, October 1, 2009 - 10:12:21 AM
Last modification on : Friday, May 29, 2020 - 4:34:13 PM
Long-term archiving on: : Monday, June 7, 2010 - 9:09:23 PM

Files

Clin_Cancer_Res_Figures.pdf
Files produced by the author(s)

Identifiers

Citation

David Azria, Mahmut Ozsahin, Andrew Kramar, Sheila Peters, David P Atencio, et al.. Single nucleotide polymorphisms, apoptosis, and the development of severe late adverse effects after radiotherapy.. Clinical Cancer Research, American Association for Cancer Research, 2008, 14 (19), pp.6284-8. ⟨10.1158/1078-0432.CCR-08-0700⟩. ⟨inserm-00337542⟩

Share

Metrics

Record views

461

Files downloads

3834