Skip to Main content Skip to Navigation
Journal articles

Role of survivin phosphorylation by aurora B in mitosis.

Marlène Delacour-Larose 1 My-Nhung Hoang Thi 1 Stefan Dimitrov 2, * Annie Molla 2, * 
* Corresponding author
2 INSERM U823, équipe 4 (Chromatine et Epigénétique)
INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
Abstract : The chromosomal protein passenger complex, a key mitotic regulator, consists of at least four proteins, INCENP, Aurora B, Survivin and Borealin. Survivin, in contrast to the other members of the chromosomal protein passenger complex (CPC), is mobile at metaphase. This protein is also phosphorylated by Aurora B at Threonine 117. In this work we have studied the role of the phosphorylation of Survivin in mitosis by using non phosphorylable T117A and phosphomimic T117E silent resistant Survivin mutants, inducible cell lines expressing these mutants and a combination of siRNA, time-lapse microscopy and FRAP analysis. Time lapse microscopy and FRAP analysis show that Survivin T117A mutant is very stably associated with centromeres and its expression induces a prometaphasic arrest in endogenous survivin depleted cells. In addition, Survivin T117A was unable to rescue the phenotypes of the endogenous survivin depleted cells. Expressed in these cells, the phosphomimic Survivin T117E mutant exhibits a very weak interaction with the centromeres and behaves as a dominant negative mutant inducing severe mitotic defects. Our data suggest that the Aurora B generated phosphorylation/dephosphorylation cycle of Survivin is required for proper proceeding of mitosis.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-00336297
Contributor : Thierry Gautier Connect in order to contact the contributor
Submitted on : Monday, March 19, 2012 - 10:55:19 AM
Last modification on : Friday, November 6, 2020 - 4:03:13 AM
Long-term archiving on: : Monday, November 26, 2012 - 11:25:20 AM

Files

TE-Cell_cycle4.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : inserm-00336297, version 1
  • PUBMED : 17671419

Collections

Citation

Marlène Delacour-Larose, My-Nhung Hoang Thi, Stefan Dimitrov, Annie Molla. Role of survivin phosphorylation by aurora B in mitosis.. Cell Cycle, Taylor & Francis, 2007, 6 (15), pp.1878-85. ⟨inserm-00336297⟩

Share

Metrics

Record views

105

Files downloads

370