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Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome.

Abstract : We studied the enrichment and distribution of the histone variant mH2A1 in the condensed inactive X (Xi) chromosome. By using highly specific antibodies against mH2A1 and stable HEK 293 cell lines expressing either green fluorescent protein (GFP)-mH2A1 or GFP-H2A, we found that the Xi chromosome contains approximately 1.5-fold more mH2A1 than the autosomes. To determine the in vivo distribution of mH2A1 along the X chromosome, we used a native chromatin immunoprecipitation-on-chip technique. DNA isolated from mH2A1-immunoprecipitated nucleosomes from either male or female mouse liver were hybridized to tiling microarrays covering 5 kb around most promoters or the entire X chromosome. The data show that mH2A1 is uniformly distributed across the entire Xi chromosome. Interestingly, a stronger mH2A1 enrichment along the pseudoautosomal X chromosome region was observed in both sexes. Our results indicate a potential role for macroH2A in large-scale chromosome structure and genome stability.
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Contributor : Thierry Gautier <>
Submitted on : Monday, November 3, 2008 - 1:22:32 PM
Last modification on : Thursday, April 15, 2021 - 11:22:02 AM
Long-term archiving on: : Tuesday, September 18, 2012 - 12:25:44 PM


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Flore Mietton, Aditya Sengupta, Annie Molla, Gisele Picchi, Sophie Barral, et al.. Weak but uniform enrichment of the histone variant macroH2A1 along the inactive X chromosome.. Molecular and Cellular Biology, American Society for Microbiology, 2009, 29 (1), pp.150-6. ⟨10.1128/MCB.00997-08⟩. ⟨inserm-00336261⟩



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