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Triple helix formation with Drosophila satellite repeats. Unexpected stabilization by copper ions.

Abstract : The Drosophila melanogaster (AAGAGAG)(n) satellite repeat represents up to 1.5% of the entire fly genome and may adopt non-B DNA structures such as pyrimidine triple helices. UV melting and electrophoretic mobility shift assay experiments were used to monitor the stability of intermolecular triple helices as a function of size, pH, and backbone or base modification. Three to four repeats of the heptanucleotide motif were sufficient to allow the formation of a stable complex, especially when modified TFOs were used. Unexpectedly, low concentrations (40-100 microM) of Cu(2+) were found to favor strongly pyrimidine triplex formation under near-physiological conditions. In contrast, a much higher magnesium concentration was required to stabilize these triplexes significantly, suggesting that copper may be an essential stabilizing factor for pyrimidine triplexes.
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Submitted on : Tuesday, October 28, 2008 - 2:35:23 PM
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Virginie Horn, Laurent Lacroix, Thierry Gautier, Masashi Takasugi, Jean-Louis Mergny, et al.. Triple helix formation with Drosophila satellite repeats. Unexpected stabilization by copper ions.. Biochemistry, American Chemical Society, 2004, 43 (35), pp.11196-205. ⟨10.1021/bi049287t⟩. ⟨inserm-00335093⟩

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