RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing. - Archive ouverte HAL Access content directly
Journal Articles Nature Genetics Year : 2004

RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing.

(1) , (1) , (1) , (2) , (1) , (1) , (2) , (1)
1
2

Abstract

RNA interference is a conserved mechanism by which double-stranded RNA is processed into short interfering RNAs (siRNAs) that can trigger both post-transcriptional and transcriptional gene silencing. In fission yeast, the RNA-induced initiation of transcriptional gene silencing (RITS) complex contains Dicer-generated siRNAs and is required for heterochromatic silencing. Here we show that RITS components, including Argonaute protein, bind to all known heterochromatic loci. At the mating-type region, RITS is recruited to the centromere-homologous repeat cenH in a Dicer-dependent manner, whereas the spreading of RITS across the entire 20-kb silenced domain, as well as its subsequent maintenance, requires heterochromatin machinery including Swi6 and occurs even in the absence of Dicer. Furthermore, our analyses suggest that RNA interference machinery operates in cis as a stable component of heterochromatic domains with RITS tethered to silenced loci by methylation of histone H3 at Lys9. This tethering promotes the processing of transcripts and generation of additional siRNAs for heterochromatin maintenance.

Dates and versions

inserm-00332376 , version 1 (20-10-2008)

Identifiers

Cite

Ken-Ichi Noma, Tomoyasu Sugiyama, Hugh Cam, Andre Verdel, Martin Zofall, et al.. RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing.. Nature Genetics, 2004, 36 (11), pp.1174-80. ⟨10.1038/ng1452⟩. ⟨inserm-00332376⟩

Collections

INSERM
61 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More