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The use of ultrasound in transfection and transgene expression.

Claire Rome 1 Roel Deckers 2 Chrit Moonen 2
1 INSERM U823, équipe 5 (cibles diagnostiques ou thérapeutiques et vectorisation de drogues dans le cancer du poumon)
INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble, Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie
Abstract : The interaction of ultrasound with tissue leads to radiation pressure, heat generation, and cavitation. These phenomena have been utilised for local gene delivery, transfection and control of expression. Specially designed nanocarriers or adapted ultrasound contrast agents can further enhance local delivery by: (1) increased permeability of cell membranes; (2) local release of genes. Biological carriers may also be used for local gene delivery. Stem cells and immune cells appear especially promising because of their homing capabilities to lesion sites. Imaging methods can be employed for pharmacodistribution and pharmacokinetics. MRI contrast agents can serve as non-invasive reporters on gene distribution when co-delivered with the gene. They can be used to label nanocarriers and cellular transport systems in gene therapy strategies such as those based on stem cells. Finally, ultrasound heating together with the use of a temperature sensitive promoter allows a local, physical, spatio-temporal control of transgene expression, in particular when combined with MRI temperature mapping for monitoring and even controlling ultrasound heating.
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https://www.hal.inserm.fr/inserm-00332076
Contributor : Amandine Hurbin <>
Submitted on : Monday, October 20, 2008 - 1:13:03 PM
Last modification on : Friday, November 6, 2020 - 4:04:17 AM

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Claire Rome, Roel Deckers, Chrit Moonen. The use of ultrasound in transfection and transgene expression.. Handb Exp Pharmacol, Vsevolod Gurevich, 2008, 2 (185 Pt 2), pp.225-43. ⟨10.1007/978-3-540-77496-9_9⟩. ⟨inserm-00332076⟩

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