Skip to Main content Skip to Navigation
Journal articles

Contraction of cultured human uterine smooth muscle cells after stimulation with endothelin-1.

Abstract : To our knowledge, the problem of how to maintain isolated smooth cells in a "contractile" phenotypic state without deviation after subculturing has yet to be resolved. The present study characterized the in vitro contractile response of human uterine smooth muscle cell to endothelin-1, which induces contractions in isolated uterine strips. Contractile effects were qualitatively investigated using silicone rubber substrata. Endothelin-1 was able to distort and reduce the wrinkles in the silicone surface. Contractions were also quantified by measuring the resulting change in the collagen lattice area. Endothelin-1 significantly increased the contractile response in a dose-dependent manner by selectively activating endothelin A receptors. When myometrial cells were cultured within collagen lattices, a microfilament-disrupting agent, cytochalasin B, abolished contractions, and no change was observed in smooth muscle alpha-actin immunostaining. Taken together, these observations show that the uterine smooth muscle cells are contractile and respond appropriately to a potent uterotonic agent. Based on these findings, a cultured uterine smooth muscle cell model, which could be used to elucidate the mechanisms controlling uterine activity, is proposed.
Document type :
Journal articles
Complete list of metadata

Cited literature [27 references]  Display  Hide  Download
Contributor : Michelle Breuiller-Fouche Connect in order to contact the contributor
Submitted on : Tuesday, September 16, 2008 - 3:16:54 PM
Last modification on : Saturday, June 25, 2022 - 8:48:25 PM
Long-term archiving on: : Saturday, November 26, 2016 - 1:25:32 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document


  • HAL Id : inserm-00322054, version 1
  • PUBMED : 12604645



Emmanuelle Dallot, Marcel Pouchelet, Nelly Gouhier, Dominique Cabrol, Françoise Ferré, et al.. Contraction of cultured human uterine smooth muscle cells after stimulation with endothelin-1.. Biology of Reproduction, Society for the Study of Reproduction, 2003, 68 (3), pp.937-42. ⟨inserm-00322054⟩



Record views