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Contraction of cultured human uterine smooth muscle cells after stimulation with endothelin-1.

Abstract : To our knowledge, the problem of how to maintain isolated smooth cells in a "contractile" phenotypic state without deviation after subculturing has yet to be resolved. The present study characterized the in vitro contractile response of human uterine smooth muscle cell to endothelin-1, which induces contractions in isolated uterine strips. Contractile effects were qualitatively investigated using silicone rubber substrata. Endothelin-1 was able to distort and reduce the wrinkles in the silicone surface. Contractions were also quantified by measuring the resulting change in the collagen lattice area. Endothelin-1 significantly increased the contractile response in a dose-dependent manner by selectively activating endothelin A receptors. When myometrial cells were cultured within collagen lattices, a microfilament-disrupting agent, cytochalasin B, abolished contractions, and no change was observed in smooth muscle alpha-actin immunostaining. Taken together, these observations show that the uterine smooth muscle cells are contractile and respond appropriately to a potent uterotonic agent. Based on these findings, a cultured uterine smooth muscle cell model, which could be used to elucidate the mechanisms controlling uterine activity, is proposed.
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https://www.hal.inserm.fr/inserm-00322054
Contributor : Michelle Breuiller-Fouche <>
Submitted on : Tuesday, September 16, 2008 - 3:16:54 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:03 AM
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  • HAL Id : inserm-00322054, version 1
  • PUBMED : 12604645

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Emmanuelle Dallot, Marcel Pouchelet, Nelly Gouhier, Dominique Cabrol, Françoise Ferré, et al.. Contraction of cultured human uterine smooth muscle cells after stimulation with endothelin-1.. Biology of Reproduction, Society for the Study of Reproduction, 2003, 68 (3), pp.937-42. ⟨inserm-00322054⟩

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