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Transfection ability and intracellular DNA pathway of nanostructured gene-delivery systems.

Abstract : Considerable efforts have been devoted to the design of structured materials with functional properties. Polyelectrolyte multilayer films are now a well-established nanostructured concept with numerous potential applications, in particular as biomaterial coatings. This technique allows the preparation of nanostructured architectures exhibiting specific properties for cell-activation control and local drug delivery. In this study, we used a multilayered system made of poly-(l-lysine)/hyaluronic acid (PLL/HA) as a reservoir for active DNA complexes with nonviral gene-delivery vectors, PLL, beta-cyclodextrin (CD), and PLL-CD. When embedded into the multilayered films, the transfection efficiencies of the DNA complexes and the cell viability were improved. The highest transfection efficiency was obtained with the PLL-CD/plasmid DNA (pDNA) complexes. We found that this high transfection efficiency was related to an efficient internalization of the complexes in the cell cytoplasm and selected nuclei domains through a nonendocytotic pathway. For the first time, we report the intracellular pathway of the pDNA in complexes incorporated into the multilayered system.
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https://www.hal.inserm.fr/inserm-00321869
Contributor : Maité Peney <>
Submitted on : Tuesday, September 16, 2008 - 10:02:19 AM
Last modification on : Thursday, April 23, 2020 - 2:26:33 PM

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Xin Zhang, Kamal Kant Sharma, Marcel Boeglin, Joelle Ogier, Didier Mainard, et al.. Transfection ability and intracellular DNA pathway of nanostructured gene-delivery systems.. Nano Letters, American Chemical Society, 2008, 8 (8), pp.2432-6. ⟨10.1021/nl801379y⟩. ⟨inserm-00321869⟩

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