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Journal Articles Nano Letters Year : 2008

Transfection ability and intracellular DNA pathway of nanostructured gene-delivery systems.

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Abstract

Considerable efforts have been devoted to the design of structured materials with functional properties. Polyelectrolyte multilayer films are now a well-established nanostructured concept with numerous potential applications, in particular as biomaterial coatings. This technique allows the preparation of nanostructured architectures exhibiting specific properties for cell-activation control and local drug delivery. In this study, we used a multilayered system made of poly-(l-lysine)/hyaluronic acid (PLL/HA) as a reservoir for active DNA complexes with nonviral gene-delivery vectors, PLL, beta-cyclodextrin (CD), and PLL-CD. When embedded into the multilayered films, the transfection efficiencies of the DNA complexes and the cell viability were improved. The highest transfection efficiency was obtained with the PLL-CD/plasmid DNA (pDNA) complexes. We found that this high transfection efficiency was related to an efficient internalization of the complexes in the cell cytoplasm and selected nuclei domains through a nonendocytotic pathway. For the first time, we report the intracellular pathway of the pDNA in complexes incorporated into the multilayered system.
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Dates and versions

inserm-00321869 , version 1 (16-09-2008)

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Xin Zhang, Kamal Kant Sharma, Marcel Boeglin, Joelle Ogier, Didier Mainard, et al.. Transfection ability and intracellular DNA pathway of nanostructured gene-delivery systems.. Nano Letters, 2008, 8 (8), pp.2432-6. ⟨10.1021/nl801379y⟩. ⟨inserm-00321869⟩
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