Skip to Main content Skip to Navigation
Journal articles

Localization of aurora A and aurora B kinases during interphase: role of the N-terminal domain.: Interphase localization of Aurora kinases A and B

Abstract : Aurora kinases possess a conserved catalytic domain (CD) and a N-terminal domain (ND) that varies in size and sequence. We have previously reported that the N-terminal domain of AuroraA (AurA) participates in the localization of the kinase to the centrosome in interphase. AuroraB (AurB) is a chromosome passenger protein and its N-terminal domain is not necessary for its localization or function during mitosis. Using various combinations of GFP-AurA and AurB protein domains we show that AurB N-terminal domain is required for nuclear localization in Xenopus XL2 cells in interphase. In human cells, however, we found both AurA and AurB kinases in the nucleus, AurA being mainly cytoplasmic and AurB mainly nuclear. Both proteins are actively excluded from the nucleus by a CRM1 dependent pathway. Interestingly, at a functional level, in interphase, every combination of Aurora kinase domains (ND-CD) rescues histone H3 Serine10 phosphorylation defect induced by AurB knockdown. This clearly indicates the presence of a functional AurA in the nucleus. However, the chimera ND-AurA/CD-AurB was much more efficient than the ND-AurB/ CD-AurA to rescue multinucleation also induced by AurB knockdown. This indicates that the catalytic domain of AurB is required to fulfill specific functions during mitosis that cannot be fulfilled by the catalytic domain of AurA, probably for localization reasons during mitosis.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00319921
Contributor : Hervé de Villemeur <>
Submitted on : Monday, March 19, 2012 - 10:54:49 AM
Last modification on : Friday, March 6, 2020 - 1:44:56 AM
Long-term archiving on: : Wednesday, June 20, 2012 - 2:20:56 AM

Files

Rannou_CC_june_2008_revized.pd...
Files produced by the author(s)

Identifiers

Citation

Yoann Rannou, Marie-Bérengère Troadec, Clotilde Petretti, Fabienne Hans, Stéphanie Dutertre, et al.. Localization of aurora A and aurora B kinases during interphase: role of the N-terminal domain.: Interphase localization of Aurora kinases A and B. Cell Cycle, Taylor & Francis, 2008, 7 (19), pp.3012-20. ⟨10.4161/cc.7.19.6718⟩. ⟨inserm-00319921⟩

Share

Metrics

Record views

645

Files downloads

1042